Influenza virus ( IFV ) A ( a ) and B ( b ) are the most common respiratory viruses in winter and spring . Detection of influenza virus and SAS - CoV - 2 nucleic acid in cases of viral pneumonia is conducive to the etiological diagnosis of viral pneumonia . This kit is used for the on - site rapid diagnosis and screen of the Severe Acute Respiratory Syndrome Coronavirus 2 ( SAS - CoV - 2 ) pneumonia and influenza virus( IFV ) A / B by multiplex direct RT - PCR method without RNA extraction .
In this kit , the specific genes of A ( a ) and B ( b ) influenza virus ( IFV ) and SAS - CoV - 2 virus and the housekeeping genes of human were detected simultaneously by multiple real - time RT - PCR based on fluorescence probe . The nucleic acid detection of SAS - CoV - 2 was located in the open reading frame ORFlab and the nucleocapsid protein N gene . Human housekeeping gene is used as internal reference IC to monitor sampling quality . At the same time , UDG enzyme antifouling scheme is used in this kit to prevent nucleic acid pollution .