Nucleic Acid Diagnostic Kit for SARS-CoV-2 (Fluorescence PCR)

Intended use: COVID-19 SARS-CoV-2 real time PCR Kit is used for the detection of specific ORFlab gene segment in Novel coronavirus in nasopharynx swab, sputum or alveolar lavage fluidly by real time PCR systems. It is auxiliary for early screening of clinical COVID-19.

Product Description: According to the novel coronavirus laboratory testing technical guideline (Fourth Edition) released by the National Health Protection Committee, the laboratory should confirm that one case is positive, and the target protein of the new crown virus ORF1ab gene in the specimen should be satisfied. The results of the real-time fluorescence RT-PCR test are positive for the target. The new coronavirus SARS-CoV-2 real time PCR Kit, based on one-step RT-PCR and Taqman technology, uses highly specific Taqman probes and specific primers that amplify SARS-CoV-2 virus specific gene fragment ORF1ab to combine with corresponding nucleic acid fragments. At the same time, the design of specific primers and probes as internal standards, using VIC/HEX fluorescent group labeling, to achieve the monitoring of sampling, extraction and detection process in the conserved region of human endogenous gene achieves closed reaction system which can effectively monitor sampling, extraction and detection process, thus effectively monitors the occurrence of false negative. Specific probes specifically bind with a section of DNA template in the PCR region, in PCR extended response, the exonuclease activity of Taq enzyme cuts the FAM fluorescent base group from the probe, thus setting the fluorescent base group free in the reaction system. The base group emits fluorescent light, which is detectable by apparatus. This achieves automatic detection of new corona virus (SARS-CoV-2) RNA in closed reaction system. Our testing kit uses dUTP-UDG enzyme anti-pollution system to degrade pollutants and reduce the occurrence of

false negative.